Indirect hemagglutination test and serum neutralization test for swine fever

Swine Fever IHA <br> <br> LI Shuchun classical swine fever like low strain after the cell culture was concentrated and purified by a health glutaraldehyde tanned sheep sensitized erythrocytes, blood indirectly made lyophilized swine fever A clotting diagnostic solution for detecting the antibody titer of porcine sputum in serum. : 376 immunized pigs and 45 non-immunized pig serum tests showed that the coincidence rate was as high as 98% compared with virus isolation. Using this method to detect the growth and decline of the antibodies to the swine fever, the results showed that after inoculation of the attenuated vaccine, the specific antibody appeared on the first 3-4 days, and the antibody titer entered the peak on the 15th day and was maintained until the inoculation. 3 months, then slowly decreased; disappeared in the u month after inoculation. Advantages: easy to operate and quick diagnosis. Disadvantages: poor specificity.

<br> <br> serum neutralization test sera diluted virus mixed with a known concentration of the different dilutions, cultures were replated cell culture, fluorescent antibody test for 18-24h after; or with attenuated vaccine classical swine fever (HCLV) Neutralization, inoculation into rabbits to observe the rabbit body temperature response, in order to determine the endpoint titer of the serum. The commonly used method in Zui in Western Europe is the Peroxidase Neutralization Test (NPLA), while in some North American and Latin American countries, the immunoperoxidase monolayer test (1PMA) method is used.

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