Leek scientific harvest in greenhouse

Winter is the peak season for leek consumption, and the market demand is large, and the supply mainly depends on greenhouse production. Leeks can grow continuously in the greenhouse. Vegetable farmers generally increase the frequency of harvesting to obtain more yield, which is likely to cause the growth of leek to be weak, the quality to be reduced, and even the disease to be aggravated. This is because leek is a perennial herb. The nutritional status of the root is the basis for the healthy growth and sustained high yield of the leek. The reasonable harvest of the leek is closely related to the nutritional status of the root. Reasonable harvesting of chives can improve the nutritional status of the chives' roots and stems, which is beneficial to promoting the steady and healthy growth of chives and ensuring the good quality and stable yield of chives.

The timing of harvesting is appropriate

In winter, the best time interval for harvesting green chives is 30-40 days. At this time, the height of the leek plant is generally 30-40 cm, and the number of leaves reaches 5-7. The content of various nutrients in the plant is also relatively high. Yields are in an optimal state. Too early harvest, the overall plant height and nutrient accumulation are not sufficient, the yield is low, and the flavor is weak; late harvest, the plant is easy to fall, the old leaves are easy to yellow, the risk of infection is increased, and the taste of leek tends to decline. When diseases such as gray mold occur in the field, if the plant height exceeds 25 cm, a variety of factors can be considered in advance, and early harvest can be carried out, which is conducive to disease prevention and control and maintains relatively stable overall yield.

When harvesting, a sharp and clean sickle should be used, and it should be carried out in the morning or evening on a sunny day, and it is best to harvest after dehumidifying the shed in the morning. The leeks in the morning are full of water, erect and upright. After the harvest, the leeks are fresh and crisp, the flavor is strong, the storage and transportation time is short, and the yield and quality are good, which is conducive to sales. It is not suitable to harvest leeks at low temperature and low sunshine, because in this kind of weather, the sheds often have high humidity and low temperature, and the leeks grow slowly and grow weakly, and the wounds after harvest are easily infected with diseases.

Stubble height should be moderate

Stubble height refers to the distance between the harvest position and the upper end of the chive bulb (gourd head). The stubble height should be moderate, neither too high nor too low, and 3-4 cm is ideal. Judging by the color of the blade, it is advisable to have a light yellow cross section. If the color is green, it means that the stubble is too high, and if it is white, it means that the stubble is too low. The stubble stubble is too high, which affects the yield and quality of the stubble leeks. The stubble is too low, which will easily damage the part of the leaf sheath basal tissue, which will delay the stubble harvest time, affect the stubble yield, and reduce the total annual output. Will lead to premature senescence and decreased resistance to disease.

Harvest times are appropriate

The harvesting times of greenhouse leeks should be determined according to plant growth, field water and fertilizer, disease and insect pest occurrence, and market demand. The best harvest is 3 winter and spring seasons, and the maximum should not exceed 4 harvests, which is conducive to enhancing plant stress resistance. In Beijing, harvesting should be stopped in mid-April, and roots will be promoted to lay the foundation for high yields in the coming winter.

Proper management before and after harvesting

Greenhouse leeks should avoid watering and fertilizing within one week before harvesting, and strengthen ventilation and dehumidification. After harvesting, the shed should be strengthened with heat preservation, and it should not be watered and fertilized before new leaves grow to 4-5 cm. These measures are very conducive to avoiding the occurrence of gray chives, which is beneficial to the healthy growth of chives, and to promote stable and high yields.

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Genetic Analyzer For MLPA

MLPA ( Multiplex Ligation-dependent Probe Amplification) method detects multiple copy number changes of genes or loci sites. Nowadays, MLPA is used to check large numbers of hereditary disorders and tumour profiling.Since Dutch Scientist Jan Schouten first invented it, the MLPA method was first published in 2002 'Nucleic Acid Research'. The principle of the MLPA is to apply the specific probe design targeting a region of interest on each sample DNA. MLPA method consists of the following steps:


Denaturation > Hybridization Ligation > PCR MLPA Amplificons Capillary Electrophoresis >Data Analysis

Mlpa Principle

PCR reaction consists of the three steps: denaturation, primer annealing and primer elongation. Those steps of PCR amplification are repeated many times. The fluorescence-labeled primers, which will go through the capillary electrophoresis to pass a detector, are incorporated into the size of the amplification products. The measured fluorescence was visualized as a peak pattern, the so-called electropherogram. The raw data from the capillary electrophoresis analyzer forms the input of the MLPA analysis.


The genetic analyzers of Superyears Gene can be used not only for Sanger sequencing but also for fragment analysis. Fragment analysis First, it obtains the fluorescence-labelled DNA fragments, performs the capillary electrophoresis using a Genetic Analyzer and compares the sample's relative size standard with the designed size standard markers through the analysis software.

The genetic analyzer of Superyears Gene can be used in the capillary electrophoresis during the process of the MLPA experiment, and the data obtained can be used for (compatible) professional analysis software.



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