Rabbit Artificial Insemination Practical Technology
In the early fall season, the condition of the female rabbit was poorly restored and the male rabbit lacked libido. It is more important to master this practical technique.
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The female rabbit should be ready
1. Sodium chloride 50ml diluted 25 μg oxytocin 3
2. Subcutaneously inject 1 ml of the progestin 3 dilution into the neck of each female rabbit to be bred.
Remarks: The purpose of the injection of placodicarb No. 3 is to ovulate ovulation in female rabbits and increase the pregnancy rate. Generally, females start estrus 4-8 hours after injection and ovulation begins 12 hours later. Therefore, the female rabbit was started to input processed semen 30 minutes after the injection.
Semen movement must be stable
(I) Preparation
(1) Making fake vagina
1 The sperma collection (person condom) is rinsed with warm water for 5-6 times, and then rinsed with sodium chloride solution 1-2 times. Then, the washed sperm collection sleeve is wiped dry with a dry clean sponge. .
2 Wash the collected sperm covers in a triangle soaked in a 42°C water-soaked sponge and stuff them into a 5 cm long, 3 cm diameter hard plastic tube (which must be strictly sterilized) and open the end of the sperm collection sleeve. After sleeving over the plastic tube, secure it with a rubber band.
(2) Preparation of diluent
1 Put the calculated sodium chloride liquid bottle into hot water and preheat it for use.
2 Prepare 36°C hot water for semen constant temperature.
3 Remove the sperm collection into warm water prepared in advance.
(II) Semen collection
1 When the sperm is collected, the left hand grasps the ears and neck of the female rabbit so that the hindquarters of the female rabbit face the cage; the right hand holds the false vagina and places the false vagina between the two hind limbs of the female rabbit. The false vagina opening is close to the vulva. Ministry and maintain a 30 degree angle with the level. After waiting for the male rabbit to climb on the hindquarters of the female rabbit and pushing out the penis, immediately insert the false vagina into the male rabbit penis. When the male rabbit's buttocks continue to shake, it will move forward, the hindquarters will contract and slide down on the side of the female rabbit. A "quick" cry was issued to indicate the end of ejaculation.
2 The female rabbit is released and the vagina is turned upside down so that the semen can flow into the spermatic duct.
Semen to check fine
1 The examination of semen should be performed at room temperature of 18-25°C.
2 The collected semen should be placed in a constant temperature water bath at 30°C.
3 Preheat the microscope stage to 36°C and use a 1040x lens for inspection.
4 Remove one drop of diluted semen and place it on a glass slide. Use a cover glass to hold the microscope.
Visual inspection
The amount of ejaculation refers to the number of semen injected at one time. Normal adult male rabbits each ejaculate is about 1ml.
Color and odor, semen milky white, cloudy and opaque, and some slightly yellow, color and turbidity is proportional to the concentration of sperm. No odor.
The normal semen pH is 6.6-7.6. If the pH is too high, the male rabbit may have a genital disorder and cannot be used.
Microscopy
The density of sperm refers to the number of sperm contained in each milliliter of semen. The greater the sperm density, the better. Check the sperm density to determine the degree of semen quality and determine the dilution factor, see the sperm density grade standards (see table above).
Sperm activity above 0.5, or more than 3, before insemination. The so-called sperm motility refers to the percentage of sperm that moves in a straight line in semen.
The morphological examination of sperm is mainly to check the rate of abnormal sperm, that is, the ratio of abnormal sperm count to total sperm count. The number of abnormal sperms in 500 spermatozoa in different visual fields was observed under a microscope of 400-600 magnifications. The abnormal semen in normal semen should not exceed 20%.
Semen dilution
Semen can not only increase the number of breeding through dilution, but also can provide sperm nutrition, neutralize the adverse effects of parasexual gland secretions on sperm, buffer the pH of semen, create a more suitable external environment for sperm, thereby increasing its vitality and prolong survival time. In order to better play the good role of male rabbits. As a short-term insemination, the commonly used diluent is sodium chloride solution. The dilution factor can be about 20 times.
1 Dilutions should be isothermal with semen.
2 The preheated (36°C) sodium chloride is slowly poured into the semen along the wall of the sperm collection, slightly shaken, and mixed until ready.
3 Place the diluted semen in warm water at 36°C until use.
Insemination to operate accurately
1 Use a sterilized rabbit-specific glass delivery device.
2 Baoding of the female rabbit: The left hand grasps the rabbit's ears and neck, and the right hand pushes the tail over the back and grabs the flesh of the tail and back. The hindquarters are turned upside down and the abdomen is fixed facing the inseminator.
3 The inseminat's left thumb is down, the index finger is on, press the vulva, turn the vulva open, and hold the glass inseminator in the right hand and gently insert it into the vagina along the vaginal wall. When it encounters resistance, pump it out and change it in another direction. Into the interpolation, insert 0.8cm is appropriate, the diluent 0. 5ml into the vagina near the mouth of the child.
4 After the insemination, the inseminator is slowly drawn out, and the female rabbit hips are patted to prevent semen reflux.
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