As we all know, if you need to store a large amount of cord blood, you must use a gas-liquid nitrogen tank to build a large-volume biological sample reservoir liquid nitrogen tank system to meet its storage requirements. This requires the perfect provision of hardware facilities. The gas-liquid nitrogen tank is not pure. a tank, also including liquid nitrogen level monitoring system, data transmission
The combination of the transmission system, the sample preservation management system, and the perfect cryopreservation system becomes a whole gas phase liquid nitrogen tank system.
The safety of cord blood stored in a gas-liquid nitrogen tank is self-evident. The cord blood has a very high requirement for storage temperature and storage environment. Only in the deep cryopreservation state can the cells be dormant, and all enzymes in the cell are inhibited. There is no ionization damage before it can be frozen for a long time. With automatic rehydration
The functional gas phase liquid nitrogen tank ensures long-term dormancy of the sample in the tank. The sample is not destroyed.
Before the experiment puts the cord blood into the liquid nitrogen tank for formal storage, in order to prevent the hematopoietic stem cells from being damaged during the cooling process and the low temperature environment, it is necessary to add a cryoprotectant to the cord blood hematopoietic stem cells before cooling, and during the cooling process, Slowly lower the temperature with a professional computer program,
After -90 ° C, it can be transferred to -196 ° C deep cryogenic liquid nitrogen for long-term cryopreservation, thus ensuring stem cell activity.
The Sichuan Cord Blood Bank in China has a world-class large-scale gas-liquid nitrogen tank for the storage of cord blood. All liquid nitrogen tanks are connected by vacuum pipes, and the terminal is connected to the outdoor 10,000-liter reserve liquid nitrogen storage tower for real-time liquid. Nitrogen supplementation. At the same time, there are closed circuit monitoring, liquid level monitoring, dual circuit power supply, oxygen
The 24-hour uninterrupted monitoring system, such as real-time monitoring of content, is strictly monitored to ensure the safety of the cord blood storage environment. More and more patients have received umbilical cord blood stem cell transplantation, and the patient's vital signs are stable after treatment, and the treatment is normal.
How long does the cord blood freeze in the gas-liquid nitrogen tank? The maximum age of cord blood storage is constantly changing. The world's longest established cord blood storage facility is only 25 years old. There is no actual data to prove how long the storage life of cord blood can last.
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In vitro and animal studies by the Broxmeyer group of American cord blood stem cell biology research experts have shown that cord blood hematopoietic stem cells that have been frozen for more than 15 years can still maintain their original in vitro proliferation, differentiation, expansion and in vivo implantation potential. In 2011, a research report published in the Blood magazine showed
It shows that after 23.5 years of freezing of human cord blood, the activity is still high. At present, the longest frozen life in China is 15 years for cord blood treatment.
Referring to the cryopreservation time of sperm cells and egg cells, scientists have put forward their own opinion that cord blood hematopoietic stem cells can also be stored for a longer period of time.
In 1987, Wien Kuhn, a business liaison manager in the city of Zandos, Australia, frozen some of his sperm in the hospital. After 21 years, he thawed the artificial insemination with his wife's egg and succeeded in giving birth to a 3.54 kg baby girl.
According to CCTV reports, a man from Australia, Alex, who used frozen sperm for 22 years and 329 days, tried artificial insemination with his wife and succeeded in giving birth to a healthy baby Xavier. The sperm that gave birth to Xavier was also recognized by the Guinness Book of World Records as the oldest sperm in the world.
From this point of view, the frozen life of cord blood will continue to refresh the existing record. The gas-liquid nitrogen tank can freeze the cord blood for a long way.
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