How to choose the right mitochondrial antibody?

Why choose Abbkine brand COX IV as a mitochondrial antibody?

We know that the use of internal reference in Western Blotting is to detect the internal reference protein by using the corresponding antibody in the WB process, so that the expression of the internal reference can be detected while detecting the target product, because the expression of the internal reference in each tissue and cell is relatively Constant, by measuring the amount of internal reference in each sample can be used to correct the loading error, so that the semi-quantitative results are more reliable. In addition, the internal reference can be used as a blank control to detect whether the protein transfer condition is complete, the entire Western Blot color development or the luminescence system is normal.

Cytochrome c oxidase (COX) is a heterologous oligomeric enzyme that usually contains 13 subunits located in the mitochondrial inner membrane and is an enzyme complex of the mitochondrial respiratory chain. The three largest subunits that form the catalytic center in cytochrome c oxidase are encoded by mitochondrial DNA, and the remaining 10 subunits, including COX IV, are encoded by genomic DNA within the nucleus. Defects in cytochrome c oxidase (COX) activity are associated with a variety of human diseases. The COX IV antibody can be used as an internal reference antibody for mitochondrial protein loading, and the target protein size is about 16 kD.

The commonly used total cellular protein is GAPDH and the cytoskeletal protein β-Actin or β-Tubulin. For some plant samples, a special plant Actin protein is required as an internal reference. When the experimental sample is only a nuclear protein, not a total cellular extract, a histone H (Histone H), or a proliferating cell nuclear antigen (PCNA) may be used as a nuclear reference antibody. However, in some special research topics on mitochondria as a microenvironment, it is necessary to extract the total mitochondrial protein and detect the target protein content. At this time, the conventional total cellular protein internal reference is not very suitable. We recommend the use of the internal reference antibody of mitochondrial total protein. For the detection of mitochondrial proteins, COX IV or VDAC1 is commonly used as an internal reference antibody, and COX IV is the most cited mitochondrial internal reference. The molecular weight of COX IV is about 16kD, which is suitable for detecting target proteins in large WB experiments.

Our group is doing research on apoptosis and mitochondria. It often studies the mitochondrial channel protein. It has been using Abbkine's COX IV antibody for WB, and its titer is quite high. ——One client of Wuhan University of Science and Technology

Mouse-derived monoclonal antibodies generally have superior specificity, stability, and potency over polyclonal antibodies. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high titer of 100 μl COX IV antibody (if the dilution ratio of WB assay is 1:4000, the final use solution is equivalent to 400 ml), 1 ml of COX IV antibody with lower potency (if the dilution ratio of WB assay is 1) : 200, the final use liquid is equivalent to 200ml) more cost-effective. Therefore, when selecting antibodies, not only the amount of antibody needs to be considered, but also the titer of the antibody, that is, the recommended dilution ratio for different applications.

Children's boots know that when doing wb, Western Blot should be used to compare the relative expression of the target protein under different conditions or in different tissues. The precondition is that the same amount of cells are loaded, and the basis of comparison is obtained. When the special expression is not high, the difference in the amount of the sample is likely to affect the analysis of the results. So you need an internal reference.

There are many imported brands that supply COX IV antibodies, such as Abcam, Santa Cruz, Cell Signaling (CST), Millipore, etc., which are related to COX IV antibodies. However, because it is not a specialized label and supplier of internal reference antibodies, it is not the best choice for cost performance. For example, Abcam, a 100μl COX IV antibody is quoted at ¥4418; CST is a 100μl COX IV antibody. The offer is ¥2997.

In addition, some domestic suppliers can also provide COX IV antibodies, but there are deficiencies in brand and quality assurance. The domestically packaged COX IV antibody is insufficient in quality stability, and its valence is also lost after being packaged. Some GAPDH antibodies originating in domestic laboratories have better detection activity, but there are gaps in application types and it is difficult to control the stability between batches, and only a small amount of domestically produced antibodies are also available in China. Polyclonal antibody. If you want to buy a COX IV mitochondrial antibody with a higher cost performance and an imported brand, then Abbkine may be one of your important considerations. We recommend Abbkine's COX IV monoclonal reference antibody Anti-COX IV Mouse Monoclonal Antibody (14Y2) (Cat. No. A01060).

The COX IV antibody is a monoclonal antibody, clone number 14Y2. The antigen is a KLH-conjugated partial sequence of the COX IV protein which is both generic and protein-specific. Abbkine's COX IV monoclonal antibody (Cat. #A02060) is 65% lower than Abcam and only half the price of CST. It can be effectively applied to the Western Bloting experiment as an internal reference for the total protein of mitochondria.

Abbkine also offers a 1ml large package and a larger custom-made COX IV mitochondrial reference antibody, which is ideal for routine testing of immunological assays in the mitochondria. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during the use, please contact your local agent Ai Meijie Technology Co., Ltd. directly. A high titer antibody not only affects the sensitivity of the experimental results, but also means a higher price/performance ratio. Abbkine's COX IV mitochondrial ginseng antibody In the conventional WB experiment, the recommended initial dilution ratio is 1:1000. Actually, in Abbkine application, there is a dilution ratio of 1:5,000 or higher, which needs to be based on customer-specific experiments. Conditions are optimized.

The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its China. The regional agent will provide customers with the most cost-effective routine immunological reagents, such as various tag antibodies, internal reference antibodies, conventional primary antibodies and a full range of secondary antibodies, antibody detection substrates and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.

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