Release date: 2010-11-08
Scientists at the University of Helsinki in Finland and the Mel Cancer Center at Johns Hopkinsky University in the United States have developed a tissue culture technique that maintains normal and cancerous prostate tissue removed during surgery for one week and is normal in the experiment. Play the function. The study was published in the November 1 issue of Cancer Research.
Typically, pathologists use paraffin-embedded methods to store body tissues, but paraffin wax kills tissue and causes rapid freezing. Many laboratories culture prostate cells in flasks, but these cells do not stick together as in the prostate. In a study led by Marikki Laiho, they cut prostate tissue specimens from 18 patients into thin slices of precise thickness to maintain good gas and growth factor exchange throughout the tissue. Later, Laiho et al. placed the tissue in a solution consisting of 64 components to maintain the biological function of the tissue. Tissue activity was ensured by verifying the presence of specific markers of prostate tissue cells in the study. Laiho et al. have used this tissue culture technique to determine the level of expression of DNA damage repair proteins.
Laiho said the tissue culture technique is an element that understands which DNA repair proteins can be activated or not activated in different parts of the prostate tissue and helps develop cancer treatments that target DNA repair proteins.
Related methods: Prostate tissue culture technology Completion: Laiho Research Group Laboratory: Department of Virology, Hartmann Institute, Helsinki University, Helsinki University, Department of Computational Biology, University of Helsinki, Department of Pathology, Hartmann Institute, University of Helsinki Clinical Chemistry Department of Urology, University of Helsinki University, Johns Hopkins University School of Medicine, Sidney Kimmel Comprehensive Cancer Center, Department of Pathology and Oncology, Karolinska Institute, Sweden
Source: Science Network
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