Waters Solutions - Detection of vanillin in infant formula

Background: Vanillin is a synthetic fragrance. According to the European Commission of Experts on February 24, 2000, large doses can cause headache, nausea, vomiting, difficulty breathing, and even damage to the liver and kidneys. Therefore, China is prohibited from adding it to the 1-stage infant formula in the period from 0 to June.
GB method: GB2760-2011 regulations on the amount of vanillin added: the maximum use of vanillin, ethyl vanillin and vanilla bean extract in larger infant and toddler formulas is 5mg/100mL 5mg/100mL and the appropriate amount according to the production needs, of which 100mL is calculated as ready-to-eat food, the production enterprise should be converted into the formula in the formula according to the proportion of the preparation;
Do not add any flavorings to infant formulas that range from 0 to 6 months. Note: Only the limits are specified in the national standard method, and there is no corresponding detection method.
Waters (Waters ®) Solution: Since the stage 0-6 months infant formula is prohibited detection and milk powder matrix is more complex, so the need for target purification, enrichment. The pretreatment method is to precipitate the milk powder after passing through the MAX column, as shown in the following table; vanillin is relatively polar and has a relatively low reversed phase retention. Waters' unique T1 column for polar compounds can be better retained. Target and good peak shape.
Protein precipitation :
After the milk powder is dissolved in water, the pH of the acetic acid is adjusted to 4.6 , centrifuged, filtered, and the pH is adjusted to 7 , and the sample is applied to the MAX column.
Oasis MAX 3cc/60mg solid phase extraction cartridge
Atlantis/XSelect HSS T3
- Specially designed C18 column
- increase the reversed retention of polar small molecules
- Separation reproducibility is good
Thank you very much for your trust and support to Waters! I look forward to your inquiry 800 820 2676 !

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